Development of a Thioredoxin‐Based Cofactor Regeneration System for NADPH‐Dependent Oxidoreductases
نویسندگان
چکیده
Nicotinamide cofactor-dependent oxidoreductases have become a valuable tool for the synthesis of high value chiral compounds. The feasibility biocatalytic processes involving these enzymes stands and falls with efficiency regeneration cofactors. In this study, we describe novel NADPH method based on natural thioredoxin electron delivery system. Thioredoxin 1 (Trx1) reductase (TR) from Thermus thermophilus were characterized dithiol-dependent reduction NADP+, revealing good catalytic activities particularly remarkable thermostability. TR/Trx1 system was then coupled two representative NADPH-dependent oxidoreductases, alcohol dehydrogenase cyclohexanone monooxygenase. Reaction conditions both systems optimized reaction yield selectivity. results demonstrate TR/Trx1-system its application as
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ژورنال
عنوان ژورنال: Chemcatchem
سال: 2022
ISSN: ['1867-3899', '1867-3880']
DOI: https://doi.org/10.1002/cctc.202101625